Measurement of cytosolic-free Ca2+ in plant tissue

McAinsh, Martin and Ng, Carl K-Y. (2013) Measurement of cytosolic-free Ca2+ in plant tissue. In: Calcium signalling protocols. Methods in Molecular Biology (3rd). Humana Press, USA, pp. 327-341. ISBN 978-1-58829-442-5

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A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2

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17 Jul 2012 10:27
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21 Nov 2022 14:42