Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota)

Smallwood, M and Worrall, D and Byass, L and Elias, L and Ashford, D and Doucet, C J and Holt, C and Telford, J and Lillford, P and Bowles, D J (1999) Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota). Biochemical Journal, 340 (2). pp. 385-391. ISSN 0264-6021

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Abstract

A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants, This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N- glycosylated, The corresponding gene is unique in the carrot genome and induced by cold, The antifreeze protein appears to be localized within the apoplast.

Item Type:
Journal Article
Journal or Publication Title:
Biochemical Journal
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1312
Subjects:
ID Code:
53552
Deposited By:
Deposited On:
19 Apr 2012 09:03
Refereed?:
Yes
Published?:
Published
Last Modified:
07 Oct 2020 01:53