Worrall, D and Elias, L and Ashford, D and Smallwood, M and Sidebottom, C and Lillford, P and Telford, J and Holt, C and Bowles, D (1998) A carrot leucine-rich-repeat protein that inhibits ice recrystallization. Science, 282 (5386). pp. 115-117. ISSN 0036-8075
Full text not available from this repository.Abstract
Many organisms adapted to live at subzero temperatures express antifreeze proteins that improve their tolerance to freezing. Although structurally diverse, all antifreeze proteins interact with ice surfaces, depress the freezing temperature of aqueous solutions, and inhibit ice crystal growth. A protein purified from carrot shares these functional features with antifreeze proteins of fish. Expression of the carrot complementary DNA in tobacco resulted in the accumulation of antifreeze activity in the apoplast of plants grown at greenhouse temperatures. The sequence of carrot antifreeze protein is similar to that of polygalacturonase inhibitor proteins and contains leucine-rich repeats.