Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis

Dillon, R. J. and Webster, G. and Weightman, A. J. and Dillon, V. M. and Blanford, S. and Charnley, A. K. (2008) Composition of Acridid gut bacterial communities as revealed by 16S rRNA gene analysis. Journal of Invertebrate Pathology, 97 (3). pp. 265-272. ISSN 0022-2011

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Abstract

The gut bacterial community from four species of feral locusts and grasshoppers was determined by denaturing gradient gel electrophoresis (DGGE) analysis of bacterial 16S rRNA gene fragments. The study revealed an effect of phase polymorphism on gut bacterial diversity in brown locusts from South Africa. A single bacterial phylotype, consistent with Citrobacter sp. dominated the gut microbiota of two sympatric populations of Moroccan and Italian locusts in Spain. There was evidence for Wollbachia sp. in the meadow grasshopper caught locally in the UK. Sequence analysis of DGGE products did not reveal evidence for unculturable bacteria and homologies suggested that bacterial species were principally Gammapoteobacteria from the family Enterobacteriaceae similar to those recorded previously in laboratory reared locusts.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Invertebrate Pathology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1100/1105
Subjects:
ID Code:
53090
Deposited By:
Deposited On:
09 Mar 2012 02:49
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 07:40