Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding

Harris, Neil and Taylor, Jane E and Roberts, Jeremy A (1994) Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding. Plant Molecular Biology, 25 (4). pp. 739-742. ISSN 0167-4412

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Abstract

A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family.

Item Type:
Journal Article
Journal or Publication Title:
Plant Molecular Biology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/aacsb/disciplinebasedresearch
Subjects:
ID Code:
52484
Deposited By:
Deposited On:
08 Feb 2012 12:02
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Mar 2020 03:10