Munkley, Jennifer and Copeland, Nikki A and Moignard, Victoria and Knight, John R P and Greaves, Erin and Ramsbottom, Simon A and Pownall, Mary E and Southgate, Jennifer and Ainscough, Justin F-X and Coverley, Dawn (2011) Cyclin E is recruited to the nuclear matrix during differentiation, but is not recruited in cancer cells. Nucleic Acids Research, 39 (7). pp. 2671-2677. ISSN 0305-1048
Full text not available from this repository.Abstract
Cyclin E supports pre-replication complex (pre-RC) assembly, while cyclin A-associated kinase activates DNA synthesis. We show that cyclin E, but not A, is mounted upon the nuclear matrix in sub-nuclear foci in differentiated vertebrate cells, but not in undifferentiated cells or cancer cells. In murine embryonic stem cells, Xenopus embryos and human urothelial cells, cyclin E is recruited to the nuclear matrix as cells differentiate and this can be manipulated in vitro. This suggests that pre-RC assembly becomes spatially restricted as template usage is defined. Furthermore, failure to become restricted may contribute to the plasticity of cancer cells.