Immunohistochemical characterization of cerebrovascular amyloid in 46 autopsied cases using antibodies to β protein and cystatin C

Maruyama, K and Ikeda, S and Ishihara, T and Allsop, D and Yanagisawa, N (1990) Immunohistochemical characterization of cerebrovascular amyloid in 46 autopsied cases using antibodies to β protein and cystatin C. Stroke; a journal of cerebral circulation, 21 (3). pp. 397-403. ISSN 0039-2499

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Abstract

Using immunohistochemical staining methods with antibodies to amyloid beta protein and human cystatin C, we examined cerebrovascular amyloid protein in the brains from 46 cases with cerebral amyloid angiopathy (seven with Alzheimer's disease, one with Down's syndrome, 18 with intracranial hemorrhage, 10 with cerebral infarction, and 10 elderly patients without any neurologic disorder). All cerebrovascular amyloid deposits in these 46 cases were consistently immunoreactive to anti-beta protein antibody. However, in nine cases some vascular walls with strong beta protein immunoreactivity also reacted less intensely with the anti-cystatin C antiserum. Of these nine cases, seven showed relatively heavy cerebrovascular amyloid deposition, and all seven had suffered a fatal subcortical hemorrhage presumably caused by cerebral amyloid angiopathy. Previous limited studies have suggested that the amyloid protein seen in elderly individuals with cerebral amyloid angiopathy is composed of beta protein. However, subcortical hemorrhage rarely occurs in such individuals. Our study shows that aged patients with different brain disorders commonly suffer from beta protein-type cerebral amyloid angiopathy, and we also suggest that the severity of beta protein-type cerebrovascular amyloid deposition is a fundamental factor in cerebral amyloid angiopathy-induced brain hemorrhage in the elderly. The nature of the cystatin C-immunoreactive substance in some of these vascular lesions is uncertain, but it might conceivably play an additional important role in the pathogenesis of brain hemorrhage in these cases.

Item Type:
Journal Article
Journal or Publication Title:
Stroke; a journal of cerebral circulation
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2705
Subjects:
?? adultagedaged, 80 and overamyloidamyloid beta-peptidesantibodiesblood vesselscerebral hemorrhagecerebral infarctioncerebrospinal fluid proteinscerebrovascular circulationcongo redcystatin ccystatinshumansimmunohistochemistrymiddle agednerve tissue protein ??
ID Code:
50856
Deposited By:
Deposited On:
08 Nov 2011 11:09
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 12:26