Fucose content of keratan sulphates from bovine articular cartilage

Tai, G H and Brown, G M and Morris, H G and Huckerby, T N and Nieduszynski, I A (1991) Fucose content of keratan sulphates from bovine articular cartilage. Biochemical Journal, 273 (2). pp. 307-310. ISSN 0264-6021

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Abstract

Alkaline-borohydride-reduced keratan sulphate chains were isolated from bovine articular cartilage (6-8-year-old animals). Nine keratan sulphate fractions of increasing molecular weight were prepared by gel-permeation chromatography on a calibrated column of TSK 30 XL. The samples were analysed for fucose and galactose contents (% by wt. of keratan sulphate) and fucose/galactose ratio. The fucose content increased with molecular size, but the galactose content remained constant. It was concluded that the alpha(1----3)-linked fucose [Thornton, Morris, Cockin, Huckerby, Nieduszynski, Carlstedt, Hardingham & Ratcliffe (1989) Biochem. J. 260, 277-282] was located within the poly-N-acetyl-lactosamine repeat sequence of articular-cartilage keratan sulphate.

Item Type:
Journal Article
Journal or Publication Title:
Biochemical Journal
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
?? animalsborohydridescarbohydratescartilage, articularcattlechromatography, gelfucosegalactosekeratan sulfatemagnetic resonance spectroscopymolecular weightbiochemistrycell biologymolecular biology ??
ID Code:
50616
Deposited By:
Deposited On:
01 Nov 2011 13:52
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 12:25