Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons

Broughton, S J and Kane, N S and Arthur, B and Yoder, M and Greenspan, R J and Robichon, A (1996) Endogenously inhibited protein kinase C in transgenic Drosophila embryonic neuroblasts down regulates the outgrowth of type I and II processes of cultured mature neurons. Journal of Cellular Biochemistry, 60 (4). pp. 584-599. ISSN 0730-2312

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Abstract

Embryonic neurons were cultured from transgenic Drosophila melanogaster expressing a highly specific pseudosubstrate inhibitor of protein kinase C (PKC). Flies homozygous for this transgene, which is under the control of the yeast UAS promoter, were crossed to flies homozygous for the yeast heat shock inducible transcription factor GAL 4. Following heat shock, the progeny express the pseudosubstrate inhibitor at high levels. This strategy, which has the advantage of avoiding the non-specific effects of drugs, was used to study the role of PKC in process growth of cultured, differentiating neuroblasts. An external gold particle labeling procedure using a cell surface antigen expressed by mature neurons and processes was used to visualize neuronal processes directly in the scanning electron microscope. We observed that cell cultures expressing a low concentration of the pseudosubstrate inhibitor showed a significant decrease in the number of type I and II processes as compared to control cultures, while the proportions of neuroblasts, ganglion mother cells (GMCs), and mature neurons in the clusters were little affected.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Cellular Biochemistry
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1312
Subjects:
?? AMINO ACID SEQUENCEANIMALSANIMALS, GENETICALLY MODIFIEDCALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASESCELL AGINGCELLS, CULTUREDCYCLIC AMP-DEPENDENT PROTEIN KINASESDOWN-REGULATIONDROSOPHILA MELANOGASTERENZYME INHIBITORSMOLECULAR SEQUENCE DATANEURONSPHOSPHORYL ??
ID Code:
49890
Deposited By:
Deposited On:
20 Sep 2011 09:53
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Sep 2023 04:00