Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies

OCKLEFORD, C D and HSI, B L and WAKELY, J and BADLEY, R A and WHYTE, A and FAULK, W P (1981) Propidium iodide as a nuclear marker in immunofluorescence. I. Use with tissue and cytoskeleton studies. Journal of Immunological Methods, 43 (3). pp. 261-267. ISSN 1872-7905

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Abstract

Some examples are given of immunofluorescence with tissue sections and microtubular cytoskeletons of cultured cells where the fluorescent dye propidium iodide (PI) has been used as marker of nuclei. The emission wave length of PI is longer than that of flourescein, making it possible to use several different and commonly available filter combinations. The use of nuclei as positional indicators is often a more suitable method than phase microscopy combined with immunofluorescence because of low background illumination against which morphology is viewed, circumventing the need for often expensive phase optics.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Immunological Methods
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2723
Subjects:
ID Code:
49288
Deposited By:
Deposited On:
05 Aug 2011 09:25
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Jan 2020 07:35