CONFOCAL AND CONVENTIONAL IMMUNOFLUORESCENT AND IMMUNOGOLD ELECTRON-MICROSCOPIC LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-IV IN HUMAN PLACENTA

NANAEV, A K and RUKOSUEV, V S and SHIRINSKY, V P and MILOVANOV, A P and DOMOGATSKY, S P and DUANCE, V C and BRADBURY, F M and YARROW, P and GARDINER, L and DLACEY, C and OCKLEFORD, C D (1991) CONFOCAL AND CONVENTIONAL IMMUNOFLUORESCENT AND IMMUNOGOLD ELECTRON-MICROSCOPIC LOCALIZATION OF COLLAGEN TYPE-III AND TYPE-IV IN HUMAN PLACENTA. Placenta, 12 (6). pp. 573-595. ISSN 0143-4004

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Abstract

Confocal and conventional indirect immunofluorescence and immunogold electron microscopic methods were applied to examine the distribution of extracellular matrix constituents (collagens types III and ITq in the villi of immature and term human placentae. The immunofluorescence study revealed that collagen type III is more distinct in the villous stroma of terns placenta as compared with that of the first trimester. Collagen type IV was detected mainly in endothelial and epithelial basement membranes and interestingly also to a certain extent in the stroma. Results obtained using immunoelectron microscopy support the proposal that collagen types III and IV are characteristic of stromal and basement membranes, respectively. Stromal collagen type IV is apparently localized in association with the interstitial types of collagen (I and III), in the villous stroma of term placenta.

Item Type:
Journal Article
Journal or Publication Title:
Placenta
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1309
Subjects:
?? basement-membrane componentsextracellular-matrixchorionic villimessenger-rnalymph-nodeproteinslamininidentificationultrastructurefibronectindevelopmental biologyobstetrics and gynaecologyreproductive medicine ??
ID Code:
49261
Deposited By:
Deposited On:
24 Aug 2011 09:06
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 12:15