Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells

Hopkins, Sally and Carter, Steven R. and Haycock, John W. and Fullwood, Nigel J. and MacNeil, Sheila and Rimmer, Stephen (2009) Sub-micron poly(N-isopropylacrylamide) particles as temperature responsive vehicles for the detachment and delivery of human cells. Soft Matter, 5 (24). pp. 4928-4937. ISSN 1744-683X

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Abstract

We describe the first example of particulate materials that can detach cultured cells and then release them intact in a temperature controlled manner. Topologically open microgels composed of water swollen highly branched polymers prepared from poly(N-isopropylacrylamide) (PNIPAM) were modified with a cell-adhesive peptide (GRGDS) to produce particles for gently detaching and then transferring cultured cells to new substrates. The particles bind to cell surface integrins on both dermal fibroblasts and endothelial cells and at temperatures above the lower critical solution temperature (34 degrees C) remove cells from their normal culture substrates. Brief (45 min) cooling of the resultant particle-cell dispersion to beneath 34 degrees C releases the cells to grow on new substrates. This avoids the need for trypsinisation to detach cells or centrifugation to collect cells post-detachment and offers a flexible approach to cell detachment and transport which is compatible with normal cell culture methodologies.

Item Type:
Journal Article
Journal or Publication Title:
Soft Matter
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
ID Code:
49101
Deposited By:
Deposited On:
22 Aug 2011 14:19
Refereed?:
Yes
Published?:
Published
Last Modified:
13 May 2020 01:37