Dunn, I.C. and Paton, I.R. and Clelland, A.K. and Sebastian, S. and Johnson, E.J. and Mcteir, L. and Windsor, D. and Sherman, A. and Sang, H. and Burt, D.W. and Tickle, C. and Davey, M.G. (2011) The chicken polydactyly (Po) locus causes allelic imbalance and ectopic expression of Shh during limb development. Developmental Dynamics, 240 (5). pp. 1163-1172. ISSN 1058-8388
Full text not available from this repository.Abstract
Point mutations in the intronic ZRS region of Lmbr1, a limb specific cis-regulatory element of Sonic hedgehog (Shh), are associated with polydactyly in humans, cats, and mice. We and others have recently mapped the dominant preaxial polydactyly (Po) locus in Silkie chickens to a single nucleotide polymorphism (SNP) in the ZRS region. Using polymorphisms in the chicken Shh sequence, we confirm that the ZRS region directly regulates Shh expression in the developing limb causing ectopic Shh expression in the anterior leg, prolonged Shh expression in the posterior limb, and allelic imbalance between wt and Slk Shh alleles in heterozygote limbs. Using Silkie legs, we have explored the consequences of increased Shh expression in the posterior leg on the patterning of the toes, and the induction of preaxial polydactyly. Developmental Dynamics 240:1163–1172, 2011