Abdelmohsen, Hend and Copeland, Nikki and Hardy, John (2023) Light responsive nanogels for ocular drug delivery. In: Controlled Release Society 2023 Annual Meeting & Expo, 2023-07-24 - 2023-07-28, Paris Hotel.
Full text not available from this repository.Abstract
Introduction: Neovascular age-related macular degeneration is one of the most common eye disorders that impair vision and is the leading cause of blindness in the elderly. A variety of systems have been developed to extend the lifetime of anti-angiogenics and here we employ light to trigger drug release, capitalizing on its tuneable wavelength, irradiance and duration of exposure to offer therapeutic effect (desired dosage, time, and location) repeatedly. Here we employ light-responsive nanoparticles as metformin (MET) delivery systems. Methods: Light-responsive polymers (using o-nitrobenzyl chemistry to conjugate MET) were synthesized and used to prepare different nanogel formulations that were characterised to assess polymer size/structure (NMR/GPC/FTIR) and particle size/charge (DLS/TEM/Zeta potential), followed by in vitro validation of drug loading/release and a cytocompatibility and tube formation assay. Results: Light-responsive drug delivery systems were successfully prepared and characterized by a variety of techniques. The nanogels had particle sizes ranging from 144±2 to 775±90 nm, zeta potential values from 45±2 to -59±5 mV, PDI from 0.24±0.01 to 0.96±0.05, drug loading from 79 ± 1 to 85 ± 3 µg/mg NPs. The light-responsive nanogels controlled MET release and enhanced its activity on human endothelial cells in vitro. Conclusion: This system would enable on-demand light-triggerable metformin release over an extended period, alleviating the need for multiple injections. Moreover, they could augment the antiangiogenic activity of MET as an alternative approach for corneal neovascularisation therapy. Acknowledgements: For financial support we thank the British Council Newton-Mosharafa Fund (Bureau ID: NMM28/20. Student ID: 35390491.). References: 1. H. A. M. Abdelmohsen, N. A. Copeland, J. G. Hardy. Light-responsive biomaterials for ocular drug delivery. Drug Delivery and Translational Research. 2022. DOI: 10.1007/s13346-022-01196-5. 2. M. D. Ashton, H. A. M. Abdelmohsen, A. Kazlauciunas, N. J. Fullwood, N. A. Copeland, R. M. Hathout, A. A. Metwally, J. G. Hardy. Light-responsive supramolecular assemblies for on demand delivery of cancer therapeutics. Submitted. 3. M. B. Sharma, Ö. Kap, H. A. M. Abdelmohsen, M. Firlak, G. R. Harper, J. E. Aaltonen, K. A. Bolland, R. Bragg, S. Deeley, E. Francis, N. Kazi, B. L. Mapley, V. Oikonomou, A. D. Aljohani, N. R. Halcovitch, D. Cheneler, V. Kilic, N. Horzum, J. G. Hardy. Polymer hydrogel-based microneedles for metformin release. Submitted.