Khaleque, M.A. and Rahman, M.A. and Hossain, S.I. and Ali, M.R. and Amin, M.R. and Saidur, R. and Aly Saad Aly, M. and Sultana, M. and Khan, M.Z.H. (2026) Polythiophene functionalized Ti3C2Tx-TiO2 nanorods composite based electrochemical sensing platform for the detection of Pseudomonas aeruginosa DMC-27b. Bioelectrochemistry, 167: 109082. ISSN 1567-5394
Full text not available from this repository.Abstract
Due to its ability to form antibiotic-resistant biofilms, Pseudomonas aeruginosa can cause serious, long-lasting infections, especially in individuals with weakened immune systems; therefore, its rapid detection is crucial for effective treatment. Herein, a polythiophene-functionalized Ti3C2Tx-TiO2 nanorods (NRs) based electrochemical capacitive biosensor is proposed for the determination of Pseudomonas aeruginosa DMC-27b. The capacitive characteristics of the modified electrode were evaluated using a combination of electrochemical methods, such as electrochemical impedance spectroscopy, cyclic voltammetry, galvanostatic discharge, and self-discharging. The incorporation of Ti3C2Tx-TiO2 NRs significantly enhanced both conductivity and charge storage, yielding a specific capacitance of 973.07 F/g. The biosensor demonstrated a sensitive detection range of 10–106 CFU/ml, with a limit of detection of 1.0 CFU/ml. This is the first report on the synthesis of Ti3C2Tx-TiO2 NRs and Ti3C2Tx-TiO2 NRs-doped PTh nanocomposites, and the development of Ti3C2Tx-TiO2 NRs@PTh based electrochemical capacitive biosensor for the detection of Pseudomonas aeruginosa. The biosensor, even with a gradual decline in phage activity, showed good recovery with promising results for detecting the superbug in real samples. Furthermore, the developed Ti3C2Tx-TiO2 NRs@PTh/Phage biosensor exhibited highly selective detection of Pseudomonas aeruginosa DMC-27b, as confirmed by host range and spot test results, and further demonstrated excellent reproducibility and stability.