Histological Changes in Human Gallbladder in Pathological Condition Including Cholecystitis and Cholelithiasis an Analytical Study

Pani, Dr JyotiPrakash and Pandey, Shubham and Pani, Dr Sankarsan and G.N.Geetha and M N Mahendrakar and Katti, Karuna (2013) Histological Changes in Human Gallbladder in Pathological Condition Including Cholecystitis and Cholelithiasis an Analytical Study. Journal Of Pharmacy, 3 (4). pp. 1-13. ISSN 2319-4219

Abstract

Present study compares histological changes in the wall of diseased human gall bladder like cholelithiasis & cholecystitis and find out the risk factors for the formation of gall stone in gallbladder. To conduct case control study, 60 normal (control group) human gallbladders & 30 abnormal (case group) samples having cholelithiasis and cholecystitis were obtained and processed. After proper processing, from each sample 3 slides were stained by H&E stain, Combined PAS-AB stain & Sudan Black B stain respectively for routine histochemistry, mucin histochemistry& lipid histochemistry study. Different histological findings were noted in the various layers of gallbladders. Epithelial hyperplasia, dysplasia with erodification of mucosa and sub epithelial haemorrhages was denoted in both cholelithiasis & cholecystitis. On the behalf of statistical evidence, the present study shows both group case and control are highly significant. Sulfomucin is predominant variable for cholelithiasis (P value 0.000) and sailomucin is predominant variable for cholecystitis (P value 0.0065).The Relative Risk of sulfomucin present or not present was 193:1(odd ratio) and the relative risk for sailomucin present or not present in cholecystitis was 105:1(odd ratio).The basic principle in gall stone formation is accumulation of lipids and hyper secretion of acidic mucin particularly sulfomucin. They alter the normal tissue pattern & can induce carcinogenic progression & metastasis. Blockage or inhibition of acid mucin release can prevent gall stone formation or the compounds which regulate sailylation and sulfation might help to inhibit gall stone formation & metastasis. Scope of this research is prevention is better than cure. KEYWORDs: Acidic mucin, epithelial lipid, immunohistochemistry, neutral mucin, RokitanskyAschoff Sinus