Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Dewar, Caroline E. and Oeljeklaus, Silke and Wenger, Christoph and Warscheid, Bettina and Schneider, Andre (2022) Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei. Journal of Biological Chemistry, 298 (4): 101829. ISSN 0021-9258

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Abstract

The mitochondrial F1Fo ATP synthase of the parasite Trypanosoma brucei has been previously studied in detail. This unusual enzyme switches direction in functionality during the life cycle of the parasite, acting as an ATP synthase in the insect stages, and as an ATPase to generate mitochondrial membrane potential in the mammalian bloodstream stages. Whereas the trypanosome F1 moiety is relatively highly conserved in structure and composition, the Fo subcomplex and the peripheral stalk have been shown to be more variable. Interestingly, a core subunit of the latter, the normally conserved subunit b, has been resistant to identification by sequence alignment or biochemical methods. Here, we identified a 17 kDa mitochondrial protein of the inner membrane, Tb927.8.3070, that is essential for normal growth, efficient oxidative phosphorylation, and membrane potential maintenance. Pull-down experiments and native PAGE analysis indicated that the protein is both associated with the F1Fo ATP synthase and integral to its assembly. In addition, its knockdown reduced the levels of Fo subunits, but not those of F1, and disturbed the cell cycle. Finally, analysis of structural homology using the HHpred algorithm showed that this protein has structural similarities to Fo subunit b of other species, indicating that this subunit may be a highly diverged form of the elusive subunit b.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Biological Chemistry
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
?? biochemistrycell biologymolecular biology ??
ID Code:
227009
Deposited By:
Deposited On:
17 Jan 2025 15:25
Refereed?:
Yes
Published?:
Published
Last Modified:
17 Jan 2025 15:25