<sup>13</sup>C-NMR spectroscopy of keratan sulphates

Huckerby, Thomas N. and Brown, Gavin M. and Nieduszynski, Ian A. (1995) <sup>13</sup>C-NMR spectroscopy of keratan sulphates. European Journal of Biochemistry, 231 (3). pp. 779-783. ISSN 1432-1033

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Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete <sup>13</sup>C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Galβ(1–4)GlcNAc(6S)β(1–3)Galβ(1–4)GlcNAc(6S)-ol, Gal(6S)β(1–4)GlcNAc(6S)β(1–3)Gal(1–4)GlcNAc(6S)-ol, Galβ(1–4)GlcNAc(6S)β(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, and Gal(6S)β(1–4)GlcNAc(6S)(1–3)Gal(6S)β(1–4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using <sup>13</sup>C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.

Item Type:
Journal Article
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European Journal of Biochemistry
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?? biochemistryge environmental sciences ??
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06 Feb 2009 15:12
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15 Jul 2024 10:06