An electrochemiluminescence biosensor based on Graphitic carbon nitride luminescence quenching for detection of AFB

Tian, D. and Wang, J. and Zhuang, Q. and Wu, S. and Yu, Y. and Ding, K. (2023) An electrochemiluminescence biosensor based on Graphitic carbon nitride luminescence quenching for detection of AFB. Food Chemistry, 404 (Pt A): 134183. ISSN 0308-8146

Text (Ding_TDY2020 Manuscript of NC ECL AFB1)
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Abstract

Based on graphite-like carbon nitride (g-CN) nanocomposites coupled with aptamer, a regenerable electrochemiluminescence (ECL) biosensor is developed for the quantitative detection of aflatoxin B 1 (AFB 1). In the existence of AFB 1, the structure of the aptamer changed into a loop, and the original ECL intensity was reduced owing to the enhancement of luminescence quenching between the ferrocene modified at the end of the aptamer and the luminescent substrate g-CN. Moreover, AFB 1 with oxidation state could also react with high energy state g-CN, leading to further reduction of the electrochemiluminescence signal. At optimum conditions, ECL intensity was decreased in linearity with an AFB 1 concentration range from 0.005 ng/mL to 10 ng/mL, and the minimum detectable concentration was down to 0.005 ng/mL, which realized trace detection demand with high sensitivity. It was selective for AFB 1 and its performance had been verified on rice samples, which indicated a promising applying prospect of non-enzymatic electrochemiluminescence AFB 1 detection.