Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation

Priestley, Megan and Hains, Anna and Mulholland, Iashia and Spijkers-Shaw, Sam and Zubkova, Olga and Dyer, Douglas and Saunders, Amy (2024) Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation. Other. bioRxiv.

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Abstract

The glycocalyx is a proteoglycan-rich layer present on the surface of all mammalian cells that is particularly prevalent on endothelial cells lining the vasculature. It has been hypothesized that the glycocalyx mediates leukocyte migration by masking adhesion molecules and reducing leukocyte adhesion to the endothelium. Leukocyte recruitment is a key driver of inflammatory diseases, including the chronic skin disease, psoriasis. Here, we show that leukocytes express heparan sulfate, an important glycocalyx component, on their cell surface which is lost in response to psoriasis-like skin inflammation, whilst endothelial heparan sulfate expression is not affected. Treatment with a heparan sulfate mimetic during psoriasis-like skin inflammation protected heparan sulfate from cleavage by heparanase and resulted in reduced leukocyte accumulation in skin, yet unexpectedly, led to increased clinical signs of inflammation due to reduced Treg numbers. These findings reshape our understanding of immune cell recruitment by revealing the presence and function of a heparan sulfate glycocalyx on immune cells and highlight the complex effects of heparanase inhibitors on the immune response in this context.

Item Type:
Monograph (Other)
Uncontrolled Keywords:
Research Output Funding/yes_externally_funded
Subjects:
?? yes - externally funded ??
ID Code:
221651
Deposited By:
Deposited On:
11 Nov 2024 17:05
Refereed?:
No
Published?:
Published
Last Modified:
13 Dec 2024 02:10