Direct and indirect approaches to identify drug modes of action

Tulloch, Lindsay B. and Menzies, Stefanie K. and Coron, Ross P. and Florence, Gordon J. and Smith, Terry K. (2018) Direct and indirect approaches to identify drug modes of action. IUBMB Life, 70 (1). pp. 9-22. ISSN 1521-6543

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Abstract

Phenotypic assays are becoming increasingly more common among drug discovery practices, expanding drug target diversity as lead compounds identified through such screens are not limited to known targets. While increasing diversity is beneficial to the drug discovery process and the fight against disease, the unknown modes of action of new lead compounds can hamper drug discovery as, in most cases, the process of lead compound optimization is made difficult due to the unknown nature of the target; blindly changing substituents can prove fruitless due to the inexhaustible number of potential combinations, and it is therefore desirable to rapidly identify the targets of lead compounds developed through phenotypic screening. In addition, leads identified through target-based screening often have off-target effects that contribute towards drug toxicity, and by identifying those secondary targets, the drugs can be improved. However, the identification of a leads mode of action is far from trivial and now represents a major bottleneck in the drug discovery pipeline. This review looks at some of the recent developments in the identification of drug modes of action, focusing on phenotype-based methods using metabolomics, proteomics, transcriptomics, and genomics to detect changes in phenotype in response to the presence of the drug, and affinity-based methods using modified/unmodified drug as bait to capture and identify targets.

Item Type:
Journal Article
Journal or Publication Title:
IUBMB Life
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1303
Subjects:
?? affinity chromatographydrug mode of actiongenomicsmetabolomicsphoto-affinity labelingproteomicstarget idtranscriptomicsbiochemistrymolecular biologygeneticsclinical biochemistrycell biology ??
ID Code:
221648
Deposited By:
Deposited On:
25 Jun 2024 08:25
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 01:20