Decreased irrigation volume, not irrigation placement, promotes accumulation of multiple hormones in cotton leaves during partial rootzone drying

Ma, Yingying and Yuan, Zuoqiang and Mithöfer, Axel and Geilfus, Christoph-Martin and Dodd, Ian C. (2024) Decreased irrigation volume, not irrigation placement, promotes accumulation of multiple hormones in cotton leaves during partial rootzone drying. Environmental and Experimental Botany, 223: 105781. ISSN 0098-8472

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Abstract

While ABA is often assumed to mediate partial stomatal closure as the soil dries, other plant hormones and hydraulic signals may also be involved. We tested whether irrigation volume (% of crop evapotranspiration, ET) and placement (partial rootzone drying [PRD] or deficit irrigation [DI], which irrigate part or all of the rootzone respectively) affect this signalling by measuring stomatal conductance (gs), leaf and shoot water potential (Ψleaf, Ψshoot), shoot xylem sap ABA concentration ([X-ABA]shoot) and various foliar hormones (ABA, IAA, SA, JA, JA-Ile and cis-OPDA) in cotton plants exposed to different irrigation volumes (100%ET or 50%ET) and placements (DI or PRD). Partial rootzone drying caused stomatal closure coincident with sustained foliar ABA accumulation and minimal changes in Ψshoot, but continued soil drying of the dry compartment reversed partial stomatal closure (with gs of 100%ET PRD plants sometimes greater than well-watered plants). With 100%ET PRD, partial stomatal closure correlated with decreased soil moisture of the dry compartment and increased [ABA]leaf, but neither Ψleaf nor [X-ABA]shoot. Irrespective of irrigation placement, 50%ET significantly decreased gs, Ψleaf and Ψshoot, but significantly increased [ABA]leaf, [X-ABA]shoot, [SA]leaf, [IAA]leaf and [cis-OPDA]leaf, with stomatal closure of 50%ET PRD plants occurring earlier than 50%ET DI plants. While stomatal closure at 50%ET correlated with foliar accumulation of multiple plant hormones, foliar ABA dynamics best explained transient stomatal closure at 100%ET PRD but not stomatal re-opening with prolonged soil drying. Thus, stomatal sensitivity to drying soil (and putative regulatory signals such as ABA) depended on irrigation volume and placement.