Utilizing river and wastewater as a SARS-CoV-2 surveillance tool in settings with limited formal sewage systems

Barnes, Kayla G. and Levy, Joshua I. and Gauld, Jillian and Rigby, Jonathan and Kanjerwa, Oscar and Uzzell, Christopher B. and Chilupsya, Chisomo and Anscombe, Catherine and Tomkins-Tinch, Christopher and Mbeti, Omar and Cairns, Edward and Thole, Herbert and McSweeney, Shannon and Chibwana, Marah G. and Ashton, Philip M. and Jere, Khuzwayo C. and Meschke, John Scott and Diggle, Peter and Cornick, Jennifer and Chilima, Benjamin and Jambo, Kondwani and Andersen, Kristian G. and Kawalazira, Gift and Paterson, Steve and Nyirenda, Tonney S. and Feasey, Nicholas (2023) Utilizing river and wastewater as a SARS-CoV-2 surveillance tool in settings with limited formal sewage systems. Nature Communications, 14 (1): 7883. ISSN 2041-1723

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Abstract

The COVID-19 pandemic has profoundly impacted health systems globally and robust surveillance has been critical for pandemic control, however not all countries can currently sustain community pathogen surveillance programs. Wastewater surveillance has proven valuable in high-income settings, but less is known about the utility of water surveillance of pathogens in low-income countries. Here we show how wastewater surveillance of SAR-CoV-2 can be used to identify temporal changes and help determine circulating variants quickly. In Malawi, a country with limited community-based COVID-19 testing capacity, we explore the utility of rivers and wastewater for SARS-CoV-2 surveillance. From May 2020–May 2022, we collect water from up to 112 river or defunct wastewater treatment plant sites, detecting SARS-CoV-2 in 8.3% of samples. Peak SARS-CoV-2 detection in water samples predate peaks in clinical cases. Sequencing of water samples identified the Beta, Delta, and Omicron variants, with Delta and Omicron detected well in advance of detection in patients. Our work highlights how wastewater can be used to detect emerging waves, identify variants of concern, and provide an early warning system in settings with no formal sewage systems.

Item Type:
Journal Article
Journal or Publication Title:
Nature Communications
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1300
Subjects:
?? general biochemistry,genetics and molecular biologygeneral chemistrygeneral physics and astronomybiochemistry, genetics and molecular biology(all)chemistry(all)physics and astronomy(all) ??
ID Code:
210942
Deposited By:
Deposited On:
04 Dec 2023 11:10
Refereed?:
Yes
Published?:
Published
Last Modified:
20 Sep 2024 10:10