Chromosome genome assembly and whole genome sequencing of 110 individuals of Conogethes punctiferalis (Guenée)

Gao, B. and Peng, Y. and Jin, M. and Zhang, L. and Han, X. and Wu, C. and Yuan, H. and Awawing, A. and Zheng, F. and Li, X. and Xiao, Y. (2023) Chromosome genome assembly and whole genome sequencing of 110 individuals of Conogethes punctiferalis (Guenée). Scientific Data, 10 (1): 805. ISSN 2052-4463

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Abstract

The yellow peach moth, Conogethes punctiferalis, is a highly polyphagous pest widespread in eastern and southern Asia. It demonstrates a unique ability to adapt to rotten host fruits and displays resistance to pathogenic microorganisms, including fungi. However, the lack of available genomic resources presents a challenge in comprehensively understanding the evolution of its innate immune genes. Here, we report a high-quality chromosome-level reference genome for C. punctiferalis utilizing PacBio HiFi sequencing and Hi-C technology. The genome assembly was 494 Mb in length with a contig N50 of 3.25 Mb. We successfully anchored 1,226 contigs to 31 pseudochromosomes. Our BUSCO analysis further demonstrated a gene coverage completeness of 96.3% in the genome assembly. Approximately 43% repeat sequences and 21,663 protein-coding genes were identified. In addition, we resequenced 110 C. punctiferalis individuals from east China, achieving an average coverage of 18.4 × and identifying 5.8 million high-quality SNPs. This work provides a crucial resource for understanding the evolutionary mechanism of C. punctiferalis’ innate immune system and will help in developing new antibacterial drugs.

Item Type:
Journal Article
Journal or Publication Title:
Scientific Data
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1000
Subjects:
?? general ??
ID Code:
210722
Deposited By:
Deposited On:
30 Nov 2023 16:55
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Dec 2023 03:30