Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting

Porter, Jonathan and Robinson, Joan and Pickup, Roger and Edwards, Clive (1995) Recovery of a bacterial sub-population from sewage using immunofluorescent flow cytometry and cell sorting. FEMS Microbiology Letters, 133 (1-2). pp. 195-199. ISSN 0378-1097

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Abstract

The effectiveness of immunofluorescence flow cytometry and cell sorting to detect, quantify and separate indigenous bacterial populations present in low concentrations in sewage outflow was investigated. Preparatory experiments for targeted recovery revealed indigenous, immunoglobulin-G-binding particles present at low levels in sewage outflow samples taken from Coniston Water. Fluorescence-activated cell sorting of this population was employed to enrich for these particles, which were confirmed as bacterial cells. This cell population comprised approximately 23% of the total plate count on MacConkey agar before cell sorting, rising to approximately 95% after sorting. These results corresponded to cell densities of less than 5% of the total plate count on R2A agar. Taxonomic tests suggested the bacterium to be Ochrobactrum anthropi.

Item Type:
Journal Article
Journal or Publication Title:
FEMS Microbiology Letters
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2400/2404
Subjects:
?? cell sortingflow cytometryochrobactrum anthropimicrobiologymolecular biologygenetics ??
ID Code:
208935
Deposited By:
Deposited On:
01 Nov 2023 14:25
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 00:29