Fluorescent probes and flow cytometry : New insights into environmental bacteriology

Porter, Jonathan and Deere, Daniel and Pickup, Roger and Edwards, Clive (1996) Fluorescent probes and flow cytometry : New insights into environmental bacteriology. Cytometry, 23 (2). pp. 91-96. ISSN 0196-4763

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Abstract

Recent trends in flow cytometry have established new techniques in bacteriology. Advances in fluorescent dye technology complement these improvements, offering probes for a variety of cellular functions. Bacterial ecology requires the application of new techniques to help answer questions unanswerable by traditional methods alone. Here we review some aspects of how coupling the two technologies has enabled researchers to directly study individual bacterial cells, and revealed the complexity and heterogeneity present in both laboratory cultures and in environmental samples. Results are discussed with respect to viability analysis, stress induced changes, specific cell detection and cell sorting.

Item Type:
Journal Article
Journal or Publication Title:
Cytometry
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2734
Subjects:
?? cell sortingenvironmental bacteriaspecific identificationviability assessmentpathology and forensic medicinebiophysicshematologyendocrinologycell biology ??
ID Code:
208922
Deposited By:
Deposited On:
02 Nov 2023 10:25
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 00:29