Porter, J. and Mobbs, K. and Hart, C. A. and Saunders, J. R. and Pickup, R. W. and Edwards, C. (1997) Detection, distribution and probable fate of Escherichia coli 0157 from asymptomatic cattle on a dairy farm. Journal of Applied Microbiology, 83 (3). pp. 297-306. ISSN 1364-5072
Full text not available from this repository.Abstract
The use of commercial anti-Escherichia coli 0157-labelled magnetic beads was investigated to improve detection of E. coli 0157 by immunomagnetic separation (IMS) from a range of environments on a dairy farm. Immunomagnetic separation proved effective for separation of target cells from laboratory mixtures and during stress in sterile and non-sterile pond water. The IMS procedure was possible with a range of samples (water, faeces, slurry, grass and soil). Non-specific binding of non-target bacterial cells proved problematic in a number of sample types. However, indigenous E. coli 0157 cells were detected from samples with a high faecal load, and only with use of IMS. Data on the probable survival and spread of the organism around the farm environment are also discussed.