Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium

Gray, N. D. and Howarth, R. and Pickup, R. W. and Jones, J. G. and Head, I. M. (2000) Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium. Applied and Environmental Microbiology, 66 (10). pp. 4518-4522. ISSN 0099-2240

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Abstract

Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

Item Type:
Journal Article
Journal or Publication Title:
Applied and Environmental Microbiology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1305
Subjects:
?? biotechnologyfood scienceecologyapplied microbiology and biotechnology ??
ID Code:
208882
Deposited By:
Deposited On:
01 Nov 2023 15:10
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Sep 2024 14:18