Pericardial and mediastinal fat-associated lymphoid clusters are rapidly activated in an alkane-induced model of systemic lupus erythematosus

Bentkowska, Karolina and Hardgrave, Alex and Iqbal, Nadia and Gresty, Laura and Marsden, Bethany and Macharia, Sheila and Jackson-Jones, Lucy (2023) Pericardial and mediastinal fat-associated lymphoid clusters are rapidly activated in an alkane-induced model of systemic lupus erythematosus. Discovery Immunology, 2 (1): kyad017. ISSN 2754-2483

[thumbnail of DSI-2023-0026.R1 AAM]
Text (DSI-2023-0026.R1 AAM)
DSI-2023-0026.R1_AAM.pdf - Accepted Version
Available under License Creative Commons Attribution.

Download (2MB)

Abstract

Summary Systemic lupus erythematosus (SLE) is an autoimmune disease predominated by auto-antibodies that recognise cellular components. Pleural involvement is the most common SLE-related lung disease. Natural antibodies are rapidly secreted by innate-like B cells following perturbation of homeostasis and are important in the early stages of immune activation. The serous cavities are home to large numbers of innate-like B cells present both within serous fluid and resident within fat-associated lymphoid clusters (FALCs). FALCs are important hubs for B-cell activation and local antibody secretion within the body cavities. Patients with SLE can develop anti-phospholipid antibodies and in rare situations develop alveolar haemorrhage. Utilising delivery of the hydrocarbon oil pristane in C57BL/6 mice as a model of SLE we identify a rapid expansion of pleural cavity B cells as early as day 3 after intra-peritoneal pristane delivery. Following pristane delivery, pericardial B1 B cells are proliferative, express the plasma-cell surface marker CD138, and secrete both innate and class-switched antibodies highlighting that this cavity niche may play an unrecognised role in the initiation of lupus pleuritis.

Item Type:
Journal Article
Journal or Publication Title:
Discovery Immunology
Subjects:
?? slefat-associated lymphoid clusterslupuspleuritispristane ??
ID Code:
204596
Deposited By:
Deposited On:
19 Sep 2023 13:35
Refereed?:
Yes
Published?:
Published
Last Modified:
01 Nov 2024 01:21