Mathiasen, D. and Røssum, L. M. and Robinson, I. M. and Burgoyne, R. D. and East, J. M. and Møller, M. and Rasmussen, H. N. and Treiman, M. (1993) Isolation of chromaffin cell thapsigargin-sensitive Ca2+ store in light microsomes from bovine adrenal medulla. International Journal of Biochemistry, 25 (5). pp. 641-652. ISSN 0020-711X
Full text not available from this repository.Abstract
1. 1. A subcellular fractionation procedure for bovine adrenal glands was designed with the aim to study the biochemical properties of Ca2+ stores in chromaffin cells. 2. 2. The thapsigargin-sensitive compartment of Ca2+ stores was found to be highly enriched in a light microsomal fraction (LMF) on a 15-30% linear sucrose gradient, and was found to be essentially devoid of contamination by plasma, mitochondrial or secretory granule membranes. 3. 3. A Ca2+-pumping ATPase was identified in this LMF as a 97 kDa protein forming an acid-stable, Ca2+-dependent, thapsigargin-sensitive phosphorylated intermediate upon incubation with [γ-32P]ATP, suggesting this protein to represent a SERCA-3 isoform of Ca2+ ATPases. 4. 4. A major 162 kDa protein, previously demonstrated in the isolated chromaffin cells, was enriched in the LMF, distributing on sucrose gradients in parallel with the thapsigargin-sensitive Ca2+ uptake. 5. 5. LMF appears to represent a part of the thapsigargin-sensitive Ca2+ store of chromaffin cells, and should be useful for further studies of the store properties at the subcellular and molecular level.