Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila

Razzaq, Azam and Robinson, Iain M. and McMahon, Harvey T. and Skepper, Jeremy N. and Su, Ya and Zelhof, Andrew C. and Jackson, Antony P. and Gay, Nicholas J. and O'Kane, Cahir J. (2001) Amphiphysin is necessary for organization of the excitation-contraction coupling machinery of muscles, but not for synaptic vesicle endocytosis in Drosophila. Genes and Development, 15 (22). pp. 2967-2979. ISSN 0890-9369

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Abstract

Amphiphysins 1 and 2 are enriched in the mammalian brain and are proposed to recruit dynamin to sites of endocytosis. Shorter amphiphysin 2 splice variants are also found ubiquitously, with an enrichment in skeletal muscle. At the Drosophila larval neuromuscular junction, amphiphysin is localized postsynaptically and amphiphysin mutants have no major defects in neurotransmission; they are also viable, but flightless. Like mammalian amphiphysin 2 in muscles, Drosophila amphiphysin does not bind clathrin, but can tubulate lipids and is localized on T-tubules. Amphiphysin mutants have a novel phenotype, a severely disorganized T-tubule/sarcoplasmic reticulum system. We therefore propose that muscle amphiphysin is not involved in clathrin-mediated endocytosis, but in the structural organization of the membrane-bound compartments of the excitation-contraction coupling machinery of muscles.

Item Type:
Journal Article
Journal or Publication Title:
Genes and Development
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1311
Subjects:
?? dlgdynaminindirect flight musclesmyopathyryanodine receptort-tubulesgeneticsdevelopmental biologygeneral medicinemedicine(all) ??
ID Code:
203317
Deposited By:
Deposited On:
12 Sep 2023 00:08
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 12:06