Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development

Koh, Tong Wey and Korolchuk, Viktor I. and Wairkar, Yogesh P. and Jiao, Wei and Evergren, Emma and Pan, Hongling and Zhou, Yi and Venken, Koen J.T. and Shupliakov, Oleg and Robinson, Iain M. and O'Kane, Cahir J. and Bellen, Hugo J. (2007) Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development. Journal of Cell Biology, 178 (2). pp. 309-322. ISSN 0021-9525

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Epidermal growth factor receptor pathway substrate clone 15 (Eps15) is a protein implicated in endocytosis, endosomal protein sorting, and cytoskeletal organization. Its role is, however, still unclear, because of reasons including limitations of dominant-negative experiments and apparent redundancy with other endocytic proteins. We generated Drosophila eps15-null mutants and show that Eps15 is required for proper synaptic bouton development and normal levels of synaptic vesicle (SV) endocytosis. Consistent with a role in SV endocytosis, Eps15 moves from the center of synaptic boutons to the periphery in response to synaptic activity. The endocytic protein, Dap160/intersectin, is a major binding partner of Eps15, and eps15 mutants phenotypically resemble dap160 mutants. Analyses of eps15 dap160 double mutants suggest that Eps15 functions in concert with Dap160 during SV endocytosis. Based on these data, we hypothesize that Eps15 and Dap160 promote the efficiency of endocytosis from the plasma membrane by maintaining high concentrations of multiple endocytic proteins, including dynamin, at synapses.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Cell Biology
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?? cell biology ??
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Deposited On:
13 Sep 2023 15:20
Last Modified:
16 Jul 2024 00:08