Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex

Arrowsmith, Rory L. and Atkin, Anthony J. and Botchway, Stanley W. and Fairlamb, Ian J. S. and Lynam, Jason M. and Moir, James W. B. and Pascu, Sofia I. and Ward, Jonathan S. and Zhang, Wei-Qiang (2015) Confocal and fluorescence lifetime imaging sheds light on the fate of a pyrene-tagged carbon monoxide-releasing Fischer carbene chromium complex. Dalton Transactions, 44 (11). pp. 4957-4962. ISSN 1477-9226

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Abstract

The synthesis of a new pyrene-containing Fischer carbene complex is described. The complex has a broad absorbance spectrum between 300 and 400 nm and, on excitation at 345 nm in CH2Cl2 solution, emission is observed at 395 and 415 nm. Emission is also observed in PBS buffer, but in this case the resulting spectra are much broader. Confocal and fluorescence lifetime imaging indicate that emission occurs on treating HeLa cells with the complex and co-localisation studies demonstrate that this is from the mitochondria and lipid-rich regions of the cell.

Item Type:
Journal Article
Journal or Publication Title:
Dalton Transactions
Uncontrolled Keywords:
Research Output Funding/yes_externally_funded
Subjects:
?? yes - externally fundedinorganic chemistry ??
ID Code:
193722
Deposited By:
Deposited On:
18 May 2023 10:15
Refereed?:
Yes
Published?:
Published
Last Modified:
15 Jul 2024 23:49