Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice

Barnes, Michael J and Aksoylar, Halil and Krebs, Philippe and Bourdeau, Tristan and Arnold, Carrie N and Xia, Yu and Khovananth, Kevin and Engel, Isaac and Sovath, Sosathya and Lampe, Kristin and Laws, Eleana and Saunders, Amy and Butcher, Geoffrey W and Kronenberg, Mitchell and Steinbrecher, Kris and Hildeman, David and Grimes, H Leighton and Beutler, Bruce and Hoebe, Kasper (2010) Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice. Journal of Immunology, 184 (7). pp. 3743-54. ISSN 0022-1767

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Abstract

Homeostatic control of the immune system involves mechanisms that ensure the self-tolerance, survival and quiescence of hematopoietic-derived cells. In this study, we demonstrate that the GTPase of immunity associated protein (Gimap)5 regulates these processes in lymphocytes and hematopoietic progenitor cells. As a consequence of a recessive N-ethyl-N-nitrosourea-induced germline mutation in the P-loop of Gimap5, lymphopenia, hepatic extramedullary hematopoiesis, weight loss, and intestinal inflammation occur in homozygous mutant mice. Irradiated fetal liver chimeric mice reconstituted with Gimap5-deficient cells lose weight and become lymphopenic, demonstrating a hematopoietic cell-intrinsic function for Gimap5. Although Gimap5-deficient CD4(+) T cells and B cells appear to undergo normal development, they fail to proliferate upon Ag-receptor stimulation although NF-kappaB, MAP kinase and Akt activation occur normally. In addition, in Gimap5-deficient mice, CD4(+) T cells adopt a CD44(high)CD62L(low)CD69(low) phenotype and show reduced IL-7ralpha expression, and T-dependent and T-independent B cell responses are abrogated. Thus, Gimap5-deficiency affects a noncanonical signaling pathway required for Ag-receptor-induced proliferation and lymphocyte quiescence. Antibiotic-treatment or the adoptive transfer of Rag-sufficient splenocytes ameliorates intestinal inflammation and weight loss, suggesting that immune responses triggered by microbial flora causes the morbidity in Gimap5-deficient mice. These data establish Gimap5 as a key regulator of hematopoietic integrity and lymphocyte homeostasis.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Immunology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2700
Subjects:
?? geneticsgeneral medicinemedicine(all) ??
ID Code:
192319
Deposited By:
Deposited On:
02 May 2023 13:25
Refereed?:
Yes
Published?:
Published
Last Modified:
16 Jul 2024 12:02