Shih, B. and Sultan, M.J. and Chaudhry, I.H. and Tan, K.T. and Johal, K.S. and Marstan, A. and Tsai, M. and Baguneid, M. and Bayat, A. (2012) Identification of biomarkers in sequential biopsies of patients with chronic wounds receiving simultaneous acute wounds:A genetic, histological, and noninvasive imaging study. Wound Repair and Regeneration, 20 (5). pp. 757-769. ISSN 1067-1927
Full text not available from this repository.Abstract
Chronic wounds are common and lead to significant patient morbidity. A better understanding of their pathogenesis and relevant biomarkers are required. We compared acute and chronic wounds in the same individual using noninvasive imaging including spectrophotometric intracutaneous analysis (SIAscopy) and full-field laser perfusion imaging. Gene expression analysis was also performed on sequential biopsies. Whole genome gene expression microarray analysis (44k), quantitative polymerase chain reaction, and immunohistochemistry were carried out to determine gene expression levels in tissue biopsies. Fifteen Caucasian patients with chronic venous ulcers had biopsies of the wound edges and simultaneously had an acute wound created on their upper arm on days 0, 7, and 14. SIAscopy revealed increased levels of melanin (p