Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor

Abd Razak, Nurul Nadiah and Cognet, Patrick and Pérès, Yolande and Gew, Lai Ti and Aroua, Mohamed Kheireddine (2022) Kinetics and hydrodynamics of Candida antartica lipase-catalyzed synthesis of glycerol dioleate (GDO) in a continuous flow packed-bed millireactor. Journal of Cleaner Production, 373: 133816. ISSN 0959-6526

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Diacylglycerols (DAG) have been widely used in many industries due to their remarkable capabilities as emulsifiers and stabilisers. However, developing a sustainable and an effective synthesis method for DAG remains a challenge. Continuous flow bio-reactor is recognized to be more productive, controllable, and reliable instrument for developing green and intensified processes. In this work, a continuous flow packed bed millireactor was employed for the synthesis of glycerol dioelate (GDO) catalyzed by immobilized lipase namely Candida antartica. Experiments were carried out to evaluate the kinetic parameters as well as to assess the internal and external mass transfer limitations. Using one-factor-at-a-time variables method, maximum oleic acid conversion and GDO selectivity were achieved at 85% and 74% respectively, at 0.15 g of lipase, 77 min of residence time with 1.6:1 molar ratio of oleic acid/glycerol. Hydrodynamic studies showed that the esterification reaction is kinetically controlled and unaffected by external and internal mass transfer. Employing Lilly–Hornby model for kinetic evaluation, K m values increased with increasing flow rates, whereas, V max appeared to be flow rate independent. Reusability tests revealed that the activity of immobilized lipase remained the same after 9 successive reaction cycles. At 11 days of operation, the stability of the lipase in the continuous packed bed millireactor decreased only 5–7%, indicating satisfying operational results and recyclability. This work may promote the enzymatic engineering synthesis of DAG, facilitating the creation of a cleaner and safer process. It has the potential to broaden the application of enzymes in continuous flow micro or millireactors.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Cleaner Production
Uncontrolled Keywords:
?? diacylglycerolprocess intensificationlilly-hornbycontinuous flowenzyme kineticsimmobilized enzymatic reactorgeneral environmental sciencestrategy and managementindustrial and manufacturing engineeringrenewable energy, sustainability and the environmentenv ??
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Deposited On:
30 Mar 2023 13:20
Last Modified:
16 Jul 2024 11:53