Spin Saturation Transfer Difference NMR (SSTD NMR): a new tool to obtain kinetic parameters of chemical exchange processes

Quirós, María Teresa and Macdonald, Colin and Angulo, Jesús and Paz, María (2016) Spin Saturation Transfer Difference NMR (SSTD NMR): a new tool to obtain kinetic parameters of chemical exchange processes. UNSPECIFIED.

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Abstract

This detailed protocol describes the new Spin Saturation Transfer Difference Nuclear Magnetic Resonance protocol (SSTD NMR), recently developed in our group to study processes of mutual-site chemical exchange that are difficult to analyze by traditional methods. As the name suggests, this method combines the Spin Saturation Transfer method used for small molecules, with the Saturation Transfer Difference (STD) NMR method employed for the study of protein-ligand interactions, by measuring transient spin saturation transfer along increasing saturation times (build-up curves) in small organic and organometallic molecules undergoing chemical exchange.

Item Type:
Other
Uncontrolled Keywords:
Data Sharing Template/no
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ID Code:
185719
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Deposited On:
03 Feb 2023 16:00
Refereed?:
No
Published?:
Published
Last Modified:
03 Feb 2023 16:00