Response to thermal and infection stresses in an American vector of visceral leishmaniasis

Martins, Kelsilandia Aguiar and Morais, Caroline S. and Broughton, Susan J. and Lazzari, Claudio R. and Bates, Paul A. and Pereira, Marcos H. and Dillon, Rod J. (2023) Response to thermal and infection stresses in an American vector of visceral leishmaniasis. Medical and Veterinary Entomology, 37 (2). pp. 238-251. ISSN 0269-283X

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Abstract

Lutzomyia longipalpis is known as one of the primary insect vectors of visceral leishmaniasis. For such ectothermic organisms, the ambient temperature is a critical life factor. However, the impact of temperature has been ignored in many induced-stress situations of the vector life. Therefore, this study explored the interaction of Lu. longipalpis with temperature by evaluating its behaviour across a thermal gradient, thermographic recordings during blood-feeding on mice, and the gene expression of heat shock proteins (HSP) when insects were exposed to extreme temperature or infected. The results showed that 72 h after blood ingestion, Lu. longipalpis became less active and preferred relatively low temperatures. However, at later stages of blood digestion, females increased their activity and remained at higher temperatures. Real-time imaging showed that the body temperature of females can adjust rapidly to the host and remain constant until the end of blood-feeding. Insects also increased the expression of HSP90(83) during blood-feeding. Our findings suggest that Lu. longipalpis interacts with temperature by using its behaviour to avoid temperature-induced physiological damage during the gonotrophic cycle. However, the expression of certain HSP might be triggered to mitigate thermal stress in situations where a behavioural response is not the best option.