Unravelling pine response to Fusarium circinatum through Raman spectroscopy

Martin, Daniel and P Santos, Ines and Monteiro, Pedro and Amaral, Joana and da Costa, Ricardo M F and Martin-Garcia, Jorge and Batista de Carvalho, Luis A E and Marques, Maria Paula M and Pinto, Gloria (2022) Unravelling pine response to Fusarium circinatum through Raman spectroscopy. Journal of Raman Spectroscopy, 53 (12). pp. 2086-2099. ISSN 0377-0486

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Pine Pitch Canker (PPC), caused by the fungus Fusarium circinatum, is associated to significant economic and ecological losses worldwide. The effectiveness of PPC monitoring, early detection in nurseries and plantations, and the identification of resistant plant material relies on the development of objective, non-destructive and cost-effective tools. This study analysed the potential of employing Raman Spectroscopy (RS) for the early detection of biochemical changes associated with PPC in Pinus spp. with different susceptibilities to F. circinatum (highly susceptible Pinus radiata vs. relatively-resistant Pinus pinea), while unveiling possible mechanisms of action on these pathosystems. Our results indicate lignin as a key constitutive component of pine resistance against PPC and thus the potential of using this technology for the selection of PPC resistant trees. Moreover, we demonstrate the power of RS-based approaches for the rapid detection of the disease in susceptible species. Early spectral variations were found in P. radiata upon inoculation with F. circinatum from 3 days post-inoculation (dpi) onwards, whereas changes in histological analysis, relative internal stem necrosis measurements, and visual disease symptoms were only visible at 6, 8, and 9-dpi, respectively. These spectral changes have been associated to cell-wall degradation and induction of phenolic compounds synthesis upon infection in P. radiata. Altogether, we believe that RS is an innovative promising tool able to reduce disease detection time in pine species and providing an appealing alternative for the development of new and eco-friendly disease control measures.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Raman Spectroscopy
Additional Information:
This is the peer reviewed version of the following article: Daniel Martín, Inês P. Santos, Pedro Monteiro, Joana Amaral, Ricardo M. F. Costa, Jorge Martín-Garcia, Luís A. E. Batista de Carvalho, Maria Paula M. Marques, Glória Pinto (2022), Unravelling response to Fusarium circinatum through Raman spectroscopy. Journal of Raman Spectroscopy. doi: 10.1002/jrs.6446 which has been published in final form at https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jrs.6446 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
Uncontrolled Keywords:
?? pine pitch cankerpinus spp., disease detectionspecies selectionplant protectionspectroscopymaterials science(all) ??
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Deposited On:
21 Sep 2022 10:15
Last Modified:
17 Dec 2023 01:57