Gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea ( Vigna unguiculata [L.] Walp.) resistance against Cowpea severe mosaic virus (CPSMV)

Varela, Anna Lidia N. and Komatsu, Setsuko and Wang, Xin and Silva, Rodolpho G.G. and Souza, Pedro Filho N. and Moreira Lobo, Ana Karla and Vasconcelos, Ilka M. and Silveira, Joaquim Albenisio Gomes and Oliveira, Jose T.A. (2017) Gel-free/label-free proteomic, photosynthetic, and biochemical analysis of cowpea ( Vigna unguiculata [L.] Walp.) resistance against Cowpea severe mosaic virus (CPSMV). Journal of Proteomics, 163. pp. 76-91. ISSN 1874-3919

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Abstract

Cowpea severe mosaic virus (CPSMV) causes significant losses in cowpea (Vigna unguiculata) production. In this present study biochemical, physiological, and proteomic analysis were done to identify pathways and defense proteins that are altered during the incompatible interaction between the cowpea genotype BRS-Marataoã and CPSMV. The leaf protein extracts from mock- (MI) and CPSMV-inoculated plantlets (V) were evaluated at 2 and 6 days post-inoculation (DPI). Data support the assumptions that increases in biochemical (high hydrogen peroxide, antioxidant enzymes, and secondary compounds) and physiological responses (high photosynthesis index and chlorophyll content), confirmed by label-free comparative proteomic approach, in which quantitative changes in proteasome proteins, proteins related to photosynthesis, redox homeostasis, regulation factors/RNA processing proteins were observed may be implicated in the resistance of BRS-Marataoã to CPSMV. This pioneering study provides information for the selection of specific pathways and proteins, altered in this incompatible relationship, which could be chosen as targets for detailed studies to advance our understanding of the molecular, physiological, and biochemistry basis of the resistance mechanism of cowpea and design approachs to engineer plants that are more productive.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Proteomics
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/1300/1304
Subjects:
ID Code:
173836
Deposited By:
Deposited On:
19 Aug 2022 09:10
Refereed?:
Yes
Published?:
Published
Last Modified:
22 Nov 2022 11:40