The interaction of styrene maleic acid copolymers with phospholipids in Langmuir monolayers, vesicles and nanodiscs:a structural study

Hall, S.C.L. and Tognoloni, C. and Campbell, R.A. and Richens, J. and O'Shea, P. and Terry, A.E. and Price, G.J. and Dafforn, T.R. and Edler, K.J. and Arnold, T. (2022) The interaction of styrene maleic acid copolymers with phospholipids in Langmuir monolayers, vesicles and nanodiscs:a structural study. Journal of Colloid and Interface Science, 625. pp. 220-236. ISSN 1095-7103

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Abstract

Hypothesis Self-assembly of amphipathic styrene maleic acid copolymers with phospholipids in aqueous solution results in the formation of ‘nanodiscs’ containing a planar segment of phospholipid bilayer encapsulated by a polymer belt. Recently, studies have reported that lipids rapidly exchange between both nanodiscs in solution and external sources of lipids. Outstanding questions remain regarding details of polymer-lipid interactions, factors influencing lipid exchange and structural effects of such exchange processes. Here, the dynamic behaviour of nanodiscs is investigated, specifically the role of membrane charge and polymer chemistry. Experiments Two model systems are investigated: fluorescently labelled phospholipid vesicles, and Langmuir monolayers of phospholipids. Using fluorescence spectroscopy and time-resolved neutron reflectometry, the membrane potential, monolayer structure and composition are monitored with respect to time upon polymer and nanodisc interactions. Findings In the presence of external lipids, polymer chains embed throughout lipid membranes, the extent of which is governed by the net membrane charge. Nanodiscs stabilised by three different polymers will all exchange lipids and polymer with monolayers to differing extents, related to the properties of the stabilising polymer belt. These results demonstrate the dynamic nature of nanodiscs which interact with the local environment and are likely to deposit both lipids and polymer at all stages of use.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Colloid and Interface Science
Additional Information:
Funding details: Engineering and Physical Sciences Research Council, EPSRC, EP/M506461/1 Funding details: Biotechnology and Biological Sciences Research Council, BBSRC, BB/M018261/1
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2500/2504
Subjects:
ID Code:
173123
Deposited By:
Deposited On:
19 Jul 2022 12:45
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Jul 2022 12:45