Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes

Hatano, Tomoyuki and Palani, Saravanan and Papatziamou, Dimitra and Souza, Diorge and Salzer, Ralf and Tamarit, Daniel and Makwana, Mehul and Potter, Antonia and Haig, Alexandra and Xu, Wenjue and Townsend, David and Rochester, David and Bellini, Dom and Hussain, Hamdi and Ettema, Thijs and Löwe, Jan and Baum, Buzz and Robinson, Nick and Balasubramanian, Mohan (2021) Characterisation of the Ubiquitin-ESCRT pathway in Asgard archaea sheds new light on origins of membrane trafficking in eukaryotes. Biorxiv.

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Abstract

The ESCRT machinery performs a critical role in membrane remodelling events in all eukaryotic cells, including in membrane trafficking, membrane repair, cytokinetic abscission, in viral egress, and in the generation of extracellular vesicles. While the machinery is complex in modern day eukaryotes, where it comprises dozens of proteins, the system has simpler and more ancient origins. Indeed, homologues of ESCRT-III and the Vps4 ATPase, the proteins that execute the final membrane scission reaction, play analogous roles in cytokinesis and potentially in extracellular vesicle formation in TACK archaea where ESCRT-I and II homologues seem to be absent. Here, we explore the phylogeny, structure, and biochemistry of homologues of the ESCRT machinery and the associated ubiquitylation system found in genome assemblies of the recently discovered Asgard archaea. In these closest living prokaryotic relatives of eukaryotes, we provide evidence for the ESCRT-I and II sub-complexes being involved in the ubiquitin-directed recruitment of ESCRT-III,_as it is in eukaryotes. This analysis suggests a pre-eukaryotic origin for the Ub-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.

Item Type:
Journal Article
Journal or Publication Title:
Biorxiv
ID Code:
171471
Deposited By:
Deposited On:
08 Jun 2022 09:15
Refereed?:
No
Published?:
Published
Last Modified:
22 Nov 2022 11:30