Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis

Foster, Michael and Brooks, William and Jahn, Philipp and Hedberg, Jesper and Andersson, Andreas and Ashton, Lorna (2022) Demonstration of a compact deep UV Raman spatial heterodyne spectrometer for biologics analysis. Journal of Biophotonics. ISSN 1864-063X

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Abstract

Monoclonal antibodies and antibody fragments are increasingly important classes of biotherapeutics. However, these products are both challenging and expensive to manufacture. New process analytical technologies used to monitor these products during their manufacture are of significant interest. Deep UV Raman spectroscopy promises to provide the required specificity and accuracy, however instruments, have historically been large and complex. In this paper, a new deep UV Raman instrument is described using a solid-state laser and a spatial heterodyne spectrometer. The instrument overcomes practical limitations of the technique and could readily be used for online measurement. A series of observations have been made of biopharmaceutical products, including immunoglobulin G and domain antibodies. Where high levels of both specificity and linearity when measuring samples of different concentration with a precision of better than 0.05 mg/mL has been demonstrated.

Item Type:
Journal Article
Journal or Publication Title:
Journal of Biophotonics
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2200
Subjects:
ID Code:
169740
Deposited By:
Deposited On:
05 May 2022 14:35
Refereed?:
Yes
Published?:
Published
Last Modified:
05 May 2022 14:35