Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration

Khoshravesh, Roxana and Lundsgaard-Nielsen, Vanessa and Sultmanis, Stefanie and Sage, Tammy L. (2017) Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration. In: Photorespiration. Methods in Molecular Biology . Humana Press, New York, pp. 243-270. ISBN 9781493972241

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Abstract

High-resolution images obtained from plant tissues processed for light microscopy, transmission electron microscopy, and immunohistochemistry have provided crucial links between plant subcellular structure and physiology during photorespiration as well as the impact of photorespiration on plant evolution and development. This chapter presents established protocols to guide researchers in the preparation of plant tissues for high-resolution imaging with a light and transmission electron microscope and detection of proteins using immunohistochemistry. Discussion of concepts and theory behind each step in the process from tissue preservation to staining of resin-embedded tissues is included to enhance the understanding of all steps in the procedure. We also include a brief protocol for quantification of cellular parameters from high-resolution images to help researchers rigorously test hypotheses.

Item Type:
Contribution in Book/Report/Proceedings
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ID Code:
164962
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Deposited On:
24 Jan 2022 15:00
Refereed?:
No
Published?:
Published
Last Modified:
03 May 2022 03:40