Synovial fluid analysis of MMP-2, MMP-9, and COX-2 as diagnostic markers for naturally occurring septic and aseptic arthritis in horses

Abdelhaleem, S.W. and Bashandy, M.M. and Salem, S.I. and Torad, F.A. and AbuBakr, H.O. and Bayoumi, M.M. (2021) Synovial fluid analysis of MMP-2, MMP-9, and COX-2 as diagnostic markers for naturally occurring septic and aseptic arthritis in horses. Comparative Clinical Pathology, 30. pp. 577-583. ISSN 1618-5641

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Abstract

The aim of this study was to explore the diagnostic value of matrix metalloproteinase (MMP)-2 and -9, and cyclooxygenase-2 (COX-2) enzymes in the synovial fluid of horses with different forms of arthritis. Thirty-two horses were involved in this study and based on the clinical, radiographic, and synovial fluid examinations, the horses were divided into three groups: control (group I; n = 12), septic arthritis (group II; n = 5), and aseptic arthritis (group III; n = 15). After routine analysis, synovial fluid was used for assessment of MMP-2 and MMP–9 activities by gelatin zymography, and COX-2 relative gene expression by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). Synovial fluid gelatin zymography showed significant gelatinolytic activity for MMP-9 in group II. COX-2 exhibited a 50-fold expression in group II and a 4.5-fold expression in group III compared to group I. In conclusion, the results confirm that MMP-9 and COX-2 detection offers an important diagnostic potential for septic and aseptic arthritis in horses.

Item Type:
Journal Article
Journal or Publication Title:
Comparative Clinical Pathology
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700/2734
Subjects:
ID Code:
163508
Deposited By:
Deposited On:
15 Dec 2021 14:00
Refereed?:
Yes
Published?:
Published
Last Modified:
04 Jan 2022 05:51