Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana

Diaz, Manuel and Hernandez-Verdeja, Tamara and Kremnev, Dmitry and Crawford, Tim and Dubreuil, Carole and Strand, Asa (2018) Redox regulation of PEP activity during seedling establishment in Arabidopsis thaliana. Nature Communications, 9. ISSN 2041-1723

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Abstract

Activation of the plastid-encoded RNA polymerase is tightly controlled and involves a network of phosphorylation and, as yet unidentified, thiol-mediated events. Here, we characterize PLASTID REDOX INSENSITIVE2, a redox-regulated protein required for full PEP-driven transcription. PRIN2 dimers can be reduced into the active monomeric form by thioredoxins through reduction of a disulfide bond. Exposure to light increases the ratio between the monomeric and dimeric forms of PRIN2. Complementation of prin2-2 with different PRIN2 protein variants demonstrates that the monomer is required for light-activated PEP-dependent transcription and that expression of the nuclear-encoded photosynthesis genes is linked to the activity of PEP. Activation of PEP during chloroplast development likely is the source of a retrograde signal that promotes nuclear LHCB expression. Thus, regulation of PRIN2 is the thiol-mediated mechanism required for full PEP activity, with PRIN2 monomerization via reduction by TRXs providing a mechanistic link between photosynthetic electron transport and activation of photosynthetic gene expression.

Item Type:
Journal Article
Journal or Publication Title:
Nature Communications
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100
Subjects:
ID Code:
160834
Deposited By:
Deposited On:
12 Oct 2021 13:25
Refereed?:
Yes
Published?:
Published
Last Modified:
14 Oct 2021 05:45