Phloroglucinol-enhanced whey protein isolate hydrogels with antimicrobial activity for tissue engineering

Platania, Varvara and Douglas, Timothy and Zubko, Mikhajlo and Ward, Danny and Pietryga, Krzysztof and Chatzinikolaidou, Maria (2021) Phloroglucinol-enhanced whey protein isolate hydrogels with antimicrobial activity for tissue engineering. Materials Science and Engineering: C, 129. ISSN 0928-4931

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Abstract

Aging populations in developed countries will increase the demand for implantable materials to support tissue regeneration. Whey Protein Isolate (WPI), derived from dairy industry by-products, can be processed into hydrogels with the following desirable properties for applications in tissue engineering: (i) ability to support adhesion and growth of cells; (ii) ease of sterilization by autoclaving and (iii) ease of incorporation of poorly water-soluble drugs with antimicrobial activity, such as phloroglucinol (PG), the fundamental phenolic subunit of marine polyphenols. In this study, WPI hydrogels were enriched with PG at concentrations between 0 and 20% w/v. PG solubilization in WPI hydrogels is far higher than in water. Enrichment with PG did not adversely affect mechanical properties, and endowed antimicrobial activity against a range of bacteria which occur in healthcare-associated infections (HAI). WPI-PG hydrogels supported the growth of, and collagen production by human dental pulp stem cells and - to a lesser extent - of osteosarcoma-derived MG-63 cells. In summary, enrichment of WPI with PG may be a promising strategy to prevent microbial contamination while still promoting stem cell attachment and growth.

Item Type:
Journal Article
Journal or Publication Title:
Materials Science and Engineering: C
Additional Information:
This is the author’s version of a work that was accepted for publication in Materials Science and Engineering: C. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Science and Engineering: C, 129, 2021 DOI: 10.1016/j.msec.2021.112412
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/3100/3104
Subjects:
ID Code:
159047
Deposited By:
Deposited On:
31 Aug 2021 13:45
Refereed?:
Yes
Published?:
Published
Last Modified:
03 Sep 2022 00:08