Analysis of pyomelanin formation

Galeb, Hanaa and Hardy, John and Taylor, Adam (2021) Analysis of pyomelanin formation. In: Analytical Research Forum 2021, 2021-06-152021-06-16, Online.

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Abstract

The accumulation of homogentisic acid (HGA) in patients with alkaptonuria is associated with the concomitant deposition of pigments contain significant amounts of polymerised HGA (polyHGA) in the bodily tissues of the patients. The polymerisation of HGA under various different conditions in vitro was investigated to attempt to understand if there is a correlation between the conditions and rate of pigment deposition in vivo. In this study, we applied a selection of different analytical chemistry techniques including: nuclear magnetic resonance spectroscopy (NMR), high-performance liquid chromatography (HPLC), gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX), UV-visible spectroscopy (UV-visible) and electron paramagnetic resonance (EPR). We observed condition-dependent polyHGA formation, and are currently correlating these findings with literature data (e.g. histological studies) to understand polyHGA pigment deposition inside specific tissues in the body.

Item Type:
Contribution to Conference (Poster)
Journal or Publication Title:
Analytical Research Forum 2021
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2700
Subjects:
ID Code:
157047
Deposited By:
Deposited On:
13 Jul 2021 16:10
Refereed?:
Yes
Published?:
Published
Last Modified:
19 Sep 2021 17:00