Using hierarchical mesoporous Mg-Al LDH as a potential model to precisely load BSA for biological application

Li, X. and Liang, L. and Tan, Y. and Huang, Q. and Yang, S. and Tian, Y. and Zhou, J. and Wu, H. (2021) Using hierarchical mesoporous Mg-Al LDH as a potential model to precisely load BSA for biological application. Journal of Micromechanics and Molecular Physics, 5 (4): 2050012. ISSN 2424-9130

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Abstract

Bovine serum albumin (BSA) is a familiar protein and often used as a model drug to test the adsorption efficiency of material, while the cytotoxicity of hybrid is overlooked. In this study, we synthesized hierarchical mesoporous flower-like Mg-Al layered double hydroxide (LDH) microspheres through anion surfactant mediated hydrothermal method. The scanning electron microscopic images demonstrated that 10S-LDH and 100S-LDH samples possessed a porous surface and the porosity structure were affected by the concentration of anionic surfactant, i.e., sodium dodecyl sulfonate (SDS). By being immersed in the BSA/phosphate buffer solution (PBS) solution, the 100S-LDH had a loading capacity about 44.6 mg/g. Compared to 1S-LDH and 10S-LDH, the SDS intercalated in 100S-LDH could form the hydrogen bond with BSA molecules and promote the adsorption efficiency. In the cytotoxicity test towards Raw264.7 cells, the hybrid of BSA and xS-LDH showed a superior biocompatibility to the raw LDH materials. The SDS intercalated Mg-Al LDH is expected to be used in the bioactive molecules delivery. © World Scientific Publishing Company