Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering

Dziadek, Michal and Charuza, Katarzyna and Kudlackova, Radmila and Aveyard, Jenny and D'Sa, Raechelle and Serafim, Andrada and Stancu, Izabela-Cristina and Iovu, Horia and Kerns, Jemma and Allinson, Sarah and Dziadek, Kinga and Szatkowski, Piotr and Cholewa-Kowalska, Katarzyna and Bacakova, Lucie and Pamula, Elzbieta and Douglas, Timothy (2021) Modification of heat-induced whey protein isolate hydrogel with highly bioactive glass particles results in promising biomaterial for bone tissue engineering. Materials and Design, 205. ISSN 0264-1275

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Abstract

This study deals with the design and comprehensive evaluation of novel hydrogels based on whey protein isolate (WPI) for tissue regeneration. So far, WPI has been considered mainly as a food industry by-product and there are very few reports on the application of WPI in tissue engineering (TE). In this work, WPI-based hydrogels were modified with bioactive glass (BG), which is commonly used as a bone substitute material. Ready-to-use, sterile hydrogels were produced by a simple technique, namely heat-induced gelation. Two different concentrations (10 and 20% w/w) of sol–gel-derived BG particles of two different sizes (2.5 and <45 µm) were compared. µCT analysis showed that hydrogels were highly porous with almost 100% pore interconnectivity. BG particles were generally homogenously distributed in the hydrogel matrix, affecting pore size, and reducing material porosity. Thermal analysis showed that the presence of BG particles in WPI matrix reduced water content in hydrogels and improved their thermal stability. BG particles decreased enzymatic degradation of the materials. The materials underwent mineralization in simulated biological fluids (PBS and SBF) and possessed high radical scavenging capacity. In vitro tests indicated that hydrogels were cytocompatible and supported MG-63 osteoblastic cell functions.

Item Type:
Journal Article
Journal or Publication Title:
Materials and Design
Uncontrolled Keywords:
/dk/atira/pure/subjectarea/asjc/2200/2210
Subjects:
ID Code:
153909
Deposited By:
Deposited On:
19 Apr 2021 09:35
Refereed?:
Yes
Published?:
Published
Last Modified:
11 May 2022 07:31